Re: getting numbers out of gel lane plots

Stu,
Try "plot profile" (Analyse->Plot Profile) on a rectangular or line selection 
of your image. This command gives you a graph with three buttons at the bottom: 
"List", "Save..." and "Copy"... These buttons will give access to the numbers 
you need. Rectangular selection will produce a better signal to noise ratio 
compared to the line selection but  you may need to rotate your image to make 
sure that your spectrum is oriented horizontally.
Regards,
Tony.
 
 A. Shmygol, MD, PhD.
Senior Lecturer (Reproductive Medicine)
Clinical Sciences Research Institute
University of Warwick Medical School
UHCW Campus
Clifford Bridge Road
Coventry   CV2 2DX
Tel.: +44(0)2476 968702
Fax: +44(0)2476968653
e-mail: a.shmygol@xxxxxxxxxxxxx 
>>> anderstu@xxxxxxxxxxxx 03/28/06 09:28pm >>>
Dear ImageJ experts -

Does anyone know how to export the numbers behind the plots ImageJ 
produces from the PLOT LANES gel command?

Some context:

I'm trying to use ImageJ to process still images as part of a video 
camera-based spectrometer.  The camera looks at the light dispersed 
by a compact disc (i.e., the rainbow for white light).  From these 
images I use the PLOT LANES feature in ImageJ to produce plots of 
light intensity vs. position on the CD.

This works well for getting quick spectra, but I'd really like to get 
a list of the numbers that ImageJ uses to make these plots for 
subsequent processing - to produce an absorption spectrum, for 
example, or for superposing / scaling several different spectra.

The TOOLS/SAVE X-Y COORDINATES function produces a list of the pixel 
coordinates and their 8-bit intensities (pretty much 0 or 255 since 
it's a line drawing).  I could process this list to get what I want, 
but this seems crazy since ImageJ must have already calculated these 
numbers to make the plots in the first place.

Is there a way?

Thanks!

Stu
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Stuart M. Anderson
Associate Professor of Physics
Augsburg College
2211 Riverside Avenue
Minneapolis, MN  55454

Tel:    (612) 330-1012
Internet:    anderstu@xxxxxxxxxxxx 

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"Saxophones are holey relics."
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