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Re: particle analysis-Feret diameter: msg#00185java.imagej
Gabriel Landini wrote: On Sunday 27 November 2005 08:57, K. Kachrimanis wrote: No, you just calculate Feret diameter for each angle. You have a vector of Feret diameters. Maximum is the max value in this vector. Minimum is min value. Mean is the average of all values. This is how Matrox Imaging Library calculates the three values. According to MIL, breadth is max Feret/min Feret. So, no mather if you have max and min Feret or max feret and Breadth... you can calculate the third parameter easily. May be do you calculate breadth in a different way? And what should one use, the centroid or the centre of mass of the particle? Note that when using the centre of mass, holes in the particle make it difficult to standardise the measurements (the same particle filled and with holes has different feret legths because the "centre" is in a different location. Sorry, but I don't understand how this affects calculation of Feret diameter. Obviously, choosing a different centre for rotation affects the way the rotated particle is represented (well, just a translation depending on the choosen centre). However, as far as I know, Feret diameter is not constrained to pass by a given centre. So, no mather which centre you use for the rotation, once your particle is rotated, you should get the same Feret diameter. This is true also for holes: Feret is calculated on the outline of the particle. So, holes have no effect on it. a "microshape" descriptor is also available (fractal dimension), I agree totally! Even something simple like circularity is measured with a large error, due to pixellisation of the particle outline and other systematic errors introduced in the image, like shadows (just try to digitize spherical objects and analyze them... you would be surprised of the results: sometimes circularity is very far from one!) 2. fractal dimension is a bit of a statistical measure, it depends of a goodness of fit to a particular model, so the "goodness" of the result needs to be monitored by some other means (for instance the r-squared of a log-log plot linear fit). When particles are all different sizes, these goodness of fit are not comparable (it is not the same to look at a 30 pixel particle than to a 30000 pixel one, in the latter there will be much larger scales that are not present in the former, and so one is not measuring the same property unless we already know that the objects are strictly fractal). I do not think that it is a good idea to do this blindly. Same to me! Best, Philippe Grosjean Cheers,
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