Re: Colocalisation analysis

Hi Guenter,

  It worked. Thank you very much.

Sincerely,
Kong Heng



-----Original Message-----
From: ImageJ Interest Group [mailto:IMAGEJ@xxxxxxxxxxxx] On Behalf Of Guenter 
Giese
Sent: Tuesday, November 15, 2005 6:24 PM
To: IMAGEJ@xxxxxxxxxxxx
Subject: AW: Colocalisation analysis


Hi Kong,

I think this is caused by the type of conversion you use.

First split the 24bit RGB images into their channels and then use the 
respective green or red channel for your analysis. Otherwise you reduce your 
brightness (intensity) values due to the conversion process.

Hope this helps

Guenter


------------------------------------------
Dr. Guenter Giese
Light Microscopy Facility Manager
Dept. of Biomedical Optics, MPI fuer Medizinische Forschung Jahnstr. 29, 
D-69120 Heidelberg, Germany Phone (+49) 6221-486-360 (Fax: -325)
e-mail: guenter.giese@xxxxxxxxxxxxxxxxxxxxxxx
http://lightmicro.mpimf-heidelberg.mpg.de
-----Ursprüngliche Nachricht-----
Von: ImageJ Interest Group [mailto:IMAGEJ@xxxxxxxxxxxx] Im Auftrag von Lee Kong 
Heng
Gesendet: Tuesday, 15 November, 2005 10:40
An: IMAGEJ@xxxxxxxxxxxx
Betreff: Colocalisation analysis