fluorescence measurement in discrete regions of moving cells

hi all,

 

I need to measure fluorescence intensities t-stackwise in different discrete
regions of cells (eg. cytosol, membrane). I would like to use the
"measurestack" plugin but my cells are moving significantly over time. Is
there any way to define ROIs in ImageJ which will be automatically
relocalized in every frame of a t-stack  by eg. a constant distance from the
edge of fluorescence which signifies the cellborder or any other means? As I
look at reasonable number of stacks any automated form of intensity
measurement would be very welcome. 

 

thanks for any hints and help!

 

regards

 

--- thorsten

 

pharmacology,

university of würzburg, germany