Subject: Re: Possible relief for tooth-aches - msg#00233

Previous Message by Date: click to view message preview

Re: Possible relief for tooth-aches

So, you're assuming that all the yogurt in the world will use your bugs?Sounds unlikely.Without refrigeration, yogurt will spoil.Try it.The lactic acid doesn't stop a lot of things, like molds, and Streptococcus. My latest science fiction novel A Twisted Garden is now available in bookstores.On Wed, Nov 18, 2009 at 6:38 PM, Nathan McCorkle <nmz787@xxxxxxxxx> wrote: On Wed, Nov 18, 2009 at 9:16 PM, Simon Quellen Field <sfield@xxxxxxxxxxx> wrote: > That peptide, and other compounds that do the same thing, are now in most > toothpastes. > They are marketed as "tartar control" and "anti-plaque" ingredients. > > People are still going to want to brush their teeth (for reasons other than > controlling S. mutans), > so it makes sense to put the ingredient there.  Putting it in yogurt doesn't > work quite as well. > When was the last time you packed yogurt in your suitcase for a business > trip? You can get yogurt anywhere, if it was all engineered, there'd be no reason to pack it. > > Some dental chewing gums have similar ingredients, and are also easily > portable. > > I'm not sure how many "underdeveloped rural communities" there are that have > refrigeration > and yogurt, but no toothpaste or chewing gum. Yogurt doesn't need refrigeration, that's the point of fermenting milk in the first place, you introduce a culture so other wild ones don't ruin your milk. > > Making the yogurt make vaccines and vitamins, now I like that idea. > No reason not to also add p1025, since anyone allergic to it would also be > allergic to > S. mutans.  But send them toothbrushes and toothpaste while you're there. > ;-) > > Notice that S. mutans and L. acidophilus both produce lactic acid. > It isn't the acid that's the problem, but the biofilm that keeps it stuck to > the teeth, > as well as the non-acid proteolytic enzymes and chelating agents that attack > the > enamel.  If you keep the bacteria from sticking to the enamel, you have > pretty much > solved the problem. > > Meredith -- the comments about how hard it is to electroporate this plasmid > into > L. delbruckii do not bode well for our efforts with other plasmids. > > My latest science fiction novel A Twisted Garden is now available in > bookstores. > > On Wed, Nov 18, 2009 at 4:51 PM, JonathanCline <jncline@xxxxxxxxx> wrote: >> >> On Nov 17, 10:29 am, Cathal Garvey <cathalgar...@xxxxxxxxx> wrote: >> > I remember being disappointed at a vapourware GMO designed to replace >> > the >> > normal bacteria in the mouth, which didn't produce acid. It would >> > outcompete >> > the local species with a toxin that didn't harm humans (apparently), but >> > its >> > metabolism was O.K. with teeth. >> > >> > One species of bacteria in the mouth accounts for something like 95% of >> > tooth decay; perhaps we could duplicate this work? :) >> >> >> http://2008.igem.org/Team:MIT >> >> Quote: >> >>  Biogurt: A Sustainable and Savory Drug Delivery System >> >> Streptococcus mutans, the main cause of dental caries, binds to >> glycoproteins on the teeth. A clinical study (Kelly CG et al.; Nature >> Biotechnol. 1999) isolated the 20aa functional segment (p1025) that >> S.mutans uses to attach to the teeth. p1025 competitively inhibits the >> binding of S.mutans, causing unharmful bacteria to grow in its place, >> preventing the recolonization of S.mutans for 90 days. >> >> We are engineering Lactobacillus bulgaricus, a bacteria commonly found >> in yogurt, to produce and secrete this peptide under a promoter >> activated by lactose. >> >> The peptide p1025 could simply be added to any food. But production of >> this peptide by L. bulgaricus is an independent process, so inserting >> the gene into live bacteria in yogurt will enable continuous >> production. Since a new batch of yogurt can be made using the bacteria >> from a small amount of the old batch, a continuous supply of teeth- >> cleaning yogurt will be available from the first successfully >> engineered batch. This could be the key to providing effective dental >> health care in underdeveloped rural communities, especially if yogurt >> is already an integral part of the diet. >> >> Also, the p1025 gene could be replaced by any other gene, so this same >> _expression_ system could be used to produce other useful peptides. >> Yogurt with modified bacteria will provide a cheap, efficient, and >> delicious way to distribute vitamins, vaccines and more. >> Results >> Characterization >> >>    * plasmid pTG262 - Last year's Edinburgh team worked with the >> plasmid pTG262 in trying to transform it into different gram-positive >> bacteria. They had not transformed it into Lactobacillus but were >> optimistic that it could be, since pTG262 is known to be able to >> replicate in strains of Lactobacillus. Dr. Chris French was very >> helpful and graciously supplied us with this plasmid. Working >> extensively with this plasmid with all of our strains of bacteria, we >> were unable to successfully transform pTG262 into Lactobacillus, and >> we have concluded that it is our opinion that pTG262 cannot be >> electroporated into Lactobacillus delbruckii. We have added >> characterization to this part's main page and user review page, here. >> >>    * p1025-This sequence codes for a short peptide, found to >> competitively inhibit binding of S.mutans to the tooth surface (CG et >> al.; Nature Biotechnol. 1999). S.mutans takes in sugars and secretes >> lactic acid, causing dental cavities, so introduction of this peptide >> into the mouth prevents colonies. This part uses the modified Silver >> BioBrick prefix and suffix to allow for protein construction. >> >> >> >> ## Jonathan Cline >> ## jcline@xxxxxxxx >> ## Mobile: +1-805-617-0223 >> ######################## >> >> -- >> >> You received this message because you are subscribed to the Google Groups >> "DIYbio" group. >> To post to this group, send email to diybio@xxxxxxxxxxxxxxxx. >> To unsubscribe from this group, send email to >> diybio+unsubscribe@xxxxxxxxxxxxxxxx. >> For more options, visit this group at >> http://groups.google.com/group/diybio?hl=. >> >> > > -- > > You received this message because you are subscribed to the Google Groups > "DIYbio" group. > To post to this group, send email to diybio@xxxxxxxxxxxxxxxx. > To unsubscribe from this group, send email to > diybio+unsubscribe@xxxxxxxxxxxxxxxx. > For more options, visit this group at > http://groups.google.com/group/diybio?hl=. > -- Nathan McCorkle Rochester Institute of Technology College of Science, Biotechnology/Bioinformatics -- You received this message because you are subscribed to the Google Groups "DIYbio" group. To post to this group, send email to diybio@xxxxxxxxxxxxxxxx. To unsubscribe from this group, send email to diybio+unsubscribe@xxxxxxxxxxxxxxxx. For more options, visit this group at http://groups.google.com/group/diybio?hl=. -- You received this message because you are subscribed to the Google Groups "DIYbio" group. To post to this group, send email to diybio@xxxxxxxxxxxxxxxxx To unsubscribe from this group, send email to diybio+unsubscribe@xxxxxxxxxxxxxxxxx For more options, visit this group at http://groups.google.com/group/diybio?hl=.

Next Message by Date: click to view message preview

Re: Moving plants and photosynthetic animals

I can imagine the instructions for use.1) Decant Fish into a drinking glass.2) Provide some high-protein food for the fish that you wouldn't object to drinking.3) Add a little of our supplied inducer signal/diuretic mix. Don't worry, it's non-toxic. 4) Leave for about ten minutes, then decant water through a gauze strainer and drink immediately.5) Return fish to more comfortable confines.6) Brush your teeth and gargle mouthwash. Endure a lasting sense of unease. -- You received this message because you are subscribed to the Google Groups "DIYbio" group. To post to this group, send email to diybio@xxxxxxxxxxxxxxxxx To unsubscribe from this group, send email to diybio+unsubscribe@xxxxxxxxxxxxxxxxx For more options, visit this group at http://groups.google.com/group/diybio?hl=.

Previous Message by Thread: click to view message preview

Re: Possible relief for tooth-aches

So, you're assuming that all the yogurt in the world will use your bugs?Sounds unlikely.Without refrigeration, yogurt will spoil.Try it.The lactic acid doesn't stop a lot of things, like molds, and Streptococcus. My latest science fiction novel A Twisted Garden is now available in bookstores.On Wed, Nov 18, 2009 at 6:38 PM, Nathan McCorkle <nmz787@xxxxxxxxx> wrote: On Wed, Nov 18, 2009 at 9:16 PM, Simon Quellen Field <sfield@xxxxxxxxxxx> wrote: > That peptide, and other compounds that do the same thing, are now in most > toothpastes. > They are marketed as "tartar control" and "anti-plaque" ingredients. > > People are still going to want to brush their teeth (for reasons other than > controlling S. mutans), > so it makes sense to put the ingredient there.  Putting it in yogurt doesn't > work quite as well. > When was the last time you packed yogurt in your suitcase for a business > trip? You can get yogurt anywhere, if it was all engineered, there'd be no reason to pack it. > > Some dental chewing gums have similar ingredients, and are also easily > portable. > > I'm not sure how many "underdeveloped rural communities" there are that have > refrigeration > and yogurt, but no toothpaste or chewing gum. Yogurt doesn't need refrigeration, that's the point of fermenting milk in the first place, you introduce a culture so other wild ones don't ruin your milk. > > Making the yogurt make vaccines and vitamins, now I like that idea. > No reason not to also add p1025, since anyone allergic to it would also be > allergic to > S. mutans.  But send them toothbrushes and toothpaste while you're there. > ;-) > > Notice that S. mutans and L. acidophilus both produce lactic acid. > It isn't the acid that's the problem, but the biofilm that keeps it stuck to > the teeth, > as well as the non-acid proteolytic enzymes and chelating agents that attack > the > enamel.  If you keep the bacteria from sticking to the enamel, you have > pretty much > solved the problem. > > Meredith -- the comments about how hard it is to electroporate this plasmid > into > L. delbruckii do not bode well for our efforts with other plasmids. > > My latest science fiction novel A Twisted Garden is now available in > bookstores. > > On Wed, Nov 18, 2009 at 4:51 PM, JonathanCline <jncline@xxxxxxxxx> wrote: >> >> On Nov 17, 10:29 am, Cathal Garvey <cathalgar...@xxxxxxxxx> wrote: >> > I remember being disappointed at a vapourware GMO designed to replace >> > the >> > normal bacteria in the mouth, which didn't produce acid. It would >> > outcompete >> > the local species with a toxin that didn't harm humans (apparently), but >> > its >> > metabolism was O.K. with teeth. >> > >> > One species of bacteria in the mouth accounts for something like 95% of >> > tooth decay; perhaps we could duplicate this work? :) >> >> >> http://2008.igem.org/Team:MIT >> >> Quote: >> >>  Biogurt: A Sustainable and Savory Drug Delivery System >> >> Streptococcus mutans, the main cause of dental caries, binds to >> glycoproteins on the teeth. A clinical study (Kelly CG et al.; Nature >> Biotechnol. 1999) isolated the 20aa functional segment (p1025) that >> S.mutans uses to attach to the teeth. p1025 competitively inhibits the >> binding of S.mutans, causing unharmful bacteria to grow in its place, >> preventing the recolonization of S.mutans for 90 days. >> >> We are engineering Lactobacillus bulgaricus, a bacteria commonly found >> in yogurt, to produce and secrete this peptide under a promoter >> activated by lactose. >> >> The peptide p1025 could simply be added to any food. But production of >> this peptide by L. bulgaricus is an independent process, so inserting >> the gene into live bacteria in yogurt will enable continuous >> production. Since a new batch of yogurt can be made using the bacteria >> from a small amount of the old batch, a continuous supply of teeth- >> cleaning yogurt will be available from the first successfully >> engineered batch. This could be the key to providing effective dental >> health care in underdeveloped rural communities, especially if yogurt >> is already an integral part of the diet. >> >> Also, the p1025 gene could be replaced by any other gene, so this same >> _expression_ system could be used to produce other useful peptides. >> Yogurt with modified bacteria will provide a cheap, efficient, and >> delicious way to distribute vitamins, vaccines and more. >> Results >> Characterization >> >>    * plasmid pTG262 - Last year's Edinburgh team worked with the >> plasmid pTG262 in trying to transform it into different gram-positive >> bacteria. They had not transformed it into Lactobacillus but were >> optimistic that it could be, since pTG262 is known to be able to >> replicate in strains of Lactobacillus. Dr. Chris French was very >> helpful and graciously supplied us with this plasmid. Working >> extensively with this plasmid with all of our strains of bacteria, we >> were unable to successfully transform pTG262 into Lactobacillus, and >> we have concluded that it is our opinion that pTG262 cannot be >> electroporated into Lactobacillus delbruckii. We have added >> characterization to this part's main page and user review page, here. >> >>    * p1025-This sequence codes for a short peptide, found to >> competitively inhibit binding of S.mutans to the tooth surface (CG et >> al.; Nature Biotechnol. 1999). S.mutans takes in sugars and secretes >> lactic acid, causing dental cavities, so introduction of this peptide >> into the mouth prevents colonies. This part uses the modified Silver >> BioBrick prefix and suffix to allow for protein construction. >> >> >> >> ## Jonathan Cline >> ## jcline@xxxxxxxx >> ## Mobile: +1-805-617-0223 >> ######################## >> >> -- >> >> You received this message because you are subscribed to the Google Groups >> "DIYbio" group. >> To post to this group, send email to diybio@xxxxxxxxxxxxxxxx. >> To unsubscribe from this group, send email to >> diybio+unsubscribe@xxxxxxxxxxxxxxxx. >> For more options, visit this group at >> http://groups.google.com/group/diybio?hl=. >> >> > > -- > > You received this message because you are subscribed to the Google Groups > "DIYbio" group. > To post to this group, send email to diybio@xxxxxxxxxxxxxxxx. > To unsubscribe from this group, send email to > diybio+unsubscribe@xxxxxxxxxxxxxxxx. > For more options, visit this group at > http://groups.google.com/group/diybio?hl=. > -- Nathan McCorkle Rochester Institute of Technology College of Science, Biotechnology/Bioinformatics -- You received this message because you are subscribed to the Google Groups "DIYbio" group. To post to this group, send email to diybio@xxxxxxxxxxxxxxxx. To unsubscribe from this group, send email to diybio+unsubscribe@xxxxxxxxxxxxxxxx. For more options, visit this group at http://groups.google.com/group/diybio?hl=. -- You received this message because you are subscribed to the Google Groups "DIYbio" group. To post to this group, send email to diybio@xxxxxxxxxxxxxxxxx To unsubscribe from this group, send email to diybio+unsubscribe@xxxxxxxxxxxxxxxxx For more options, visit this group at http://groups.google.com/group/diybio?hl=.

Next Message by Thread: click to view message preview

Re: Possible relief for tooth-aches

Indeed; Raw Milk can spoil in a matter of hours and shouldn't be considered safe for consumption by today's standards after a day's keeping. For that matter it's not especially safe within that timeframe either. Pasteurisation or fermentation are your only options for keeping it any longer, and that's really why Yoghurt was invented in the first place. Considering the ease with which new batches of Yoghurt can be made, assuming access to milk, this is a practical idea for dental hygiene in areas where toothpaste/toothbrushes mightn't be commonplace. Having a Yeast produce the peptide would probably be more practical though for these purposes; the Cattle needed to produce the milk will do more harm than good due to their water and feeding requirements, assuming we're talking about an impoverished community. If you could ferment a drink with this peptide (and perhaps a few other useful ones) you'd be providing A) A safer source of drinking water in the form of a fermented drink and B) Whatever it is you're trying to provide, be it toothkind peptides or Vitamin A. Why do you suppose they had so much trouble transforming these plasmids? I thought plasmid transformation would be routine.. Did he suggest there that the precursor plasmid works but the peptide-carrying version doesn't? That implies toxicity to me.. -- letters.cunningprojects.comtwitter.com/onetruecathalKiva.org - Loans That Change Lives -- You received this message because you are subscribed to the Google Groups "DIYbio" group. To post to this group, send email to diybio@xxxxxxxxxxxxxxxxx To unsubscribe from this group, send email to diybio+unsubscribe@xxxxxxxxxxxxxxxxx For more options, visit this group at http://groups.google.com/group/diybio?hl=.